3LY3
Crystal Structure of fluorophore-labeled Class A Beta-lactamase PenP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Collection date | 2007-07-08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.438, 74.936, 79.034 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 1.800 |
| R-factor | 0.2236 |
| Rwork | 0.222 |
| R-free | 0.26083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4blm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.786 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.400 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.059 |
| Number of reflections | 33021 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
