3LXZ
Structure of probable Glutathione S-transferase(PP0183) from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.047, 66.358, 88.659 |
| Unit cell angles | 89.96, 85.27, 87.04 |
Refinement procedure
| Resolution | 50.000 - 1.760 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22900 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.519 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 4.780 | 1.760 |
| Rmerge | 0.091 | 0.071 | 0.426 |
| Number of reflections | 98088 | ||
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 91.4 | 98.1 | 93.7 |
| Redundancy | 3.1 | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 1.1M Ammonium(di-) Tartrate pH 7, Vapor diffusion, Sitting drop, temperature 298K |
