3LXU
Crystal Structure of Tripeptidyl Peptidase 2 (TPP II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Detector technology | CCD |
| Collection date | 2007-08-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979527 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 126.372, 126.372, 213.983 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.140 |
| Rwork | 0.241 |
| R-free | 0.29300 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.250 |
| High resolution limit [Å] | 3.140 | 3.140 |
| Number of reflections | 30956 | |
| Completeness [%] | 98.9 | 89.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 300 | 4% PEG 2000, 10mM BaCl2, 100mM HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
