3LXF
Crystal Structure of [2Fe-2S] Ferredoxin Arx from Novosphingobium aromaticivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-07-21 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.554, 78.788, 89.200 |
| Unit cell angles | 90.00, 125.04, 90.00 |
Refinement procedure
| Resolution | 28.160 - 2.300 |
| R-factor | 0.20184 |
| Rwork | 0.200 |
| R-free | 0.23929 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.451 |
| Number of reflections | 34610 | |
| <I/σ(I)> | 22.8 | 2.5 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES, 1.4M sodium citrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
