3LXD
Crystal Structure of Ferredoxin Reductase ArR from Novosphingobium aromaticivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-04-12 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.071, 140.071, 52.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.010 - 2.500 |
| R-factor | 0.19199 |
| Rwork | 0.189 |
| R-free | 0.24430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q1r |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.125 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.455 |
| Number of reflections | 18500 | |
| <I/σ(I)> | 21.1 | 3.6 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 291 | 0.1M HEPES, 17% PEG 8000, 0.1M potassium phosphate, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
