3LX1
Crystal Structure analysis of PCNA1 from Thermococcus kodakaraensis tk0535
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-08-14 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54 |
Spacegroup name | P 63 |
Unit cell lengths | 89.230, 89.230, 62.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.210 - 2.000 |
R-factor | 0.202 |
Rwork | 0.199 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.771 |
Data reduction software | CrystalClear |
Data scaling software | d*TREK (9.7 W8RSSI) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.210 | 29.210 | 2.070 |
High resolution limit [Å] | 2.000 | 4.300 | 2.000 |
Rmerge | 0.094 | 0.040 | 0.516 |
Total number of observations | 11188 | 10523 | |
Number of reflections | 19294 | ||
<I/σ(I)> | 7.9 | 23.5 | 2.2 |
Completeness [%] | 99.8 | 99.8 | 99.2 |
Redundancy | 5.57 | 5.58 | 5.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 2.4-2.8 M ammonium sulfate, 100 mM sodium citrate, 5-10% 2,4-methyl pentanediol. The protein solution has 10% glycerol, VAPOR DIFFUSION, temperature 295K |