3LX0
Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.745, 60.379, 34.531 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 24.450 - 1.500 |
| R-factor | 0.183 |
| Rwork | 0.179 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.865 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.3) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 1.480 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.067 | 0.300 |
| Number of reflections | 21820 | |
| <I/σ(I)> | 16.4 | 6.918 |
| Completeness [%] | 98.3 | 93.4 |
| Redundancy | 6.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 38% MPD, 25mM Potassium Phosphate, Calcium Chloride, pdTp, pH 6.0, vapor diffusion, hanging drop, temperature 277K |
