3LWV

Structure of H/ACA RNP bound to a substrate RNA containing 2'-deoxyuridine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	186.838, 64.326, 83.362
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.639 - 2.499
R-factor	0.2039
Rwork	0.202
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.241
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.499	2.500
Number of reflections	34570
Completeness [%]	96.8	81.1
Redundancy	11.8	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP