3LWT
Crystal structure of the Yeast Sac1: Implications for its phosphoinositide phosphatase function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9789 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 86.158, 94.732, 155.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.180 - 1.956 |
| R-factor | 0.20111 |
| Rwork | 0.199 |
| R-free | 0.24295 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.068 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.200 |
| High resolution limit [Å] | 1.970 |
| Rmerge | 0.065 |
| Number of reflections | 43565 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5 M NaCl, 0.55 M sodium Citrate, pH 7.5., VAPOR DIFFUSION, HANGING DROP, temperature 298K |
