3LWR

Structure of H/ACA RNP bound to a substrate RNA containing 4SU

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-26
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	187.355, 64.434, 83.388
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.694 - 2.203
R-factor	0.2234
Rwork	0.222
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.174
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	51780
Completeness [%]	99.7	100
Redundancy	10.2	9.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP