3LWN
Shigella IpgB2 in complex with human RhoA, GDP and Mg2+ (complex B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-06 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.530, 95.700, 102.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.130 - 2.280 |
| R-factor | 0.209 |
| Rwork | 0.204 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lw8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.661 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.130 | 2.340 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.134 | 0.654 |
| Number of reflections | 34100 | 2183 |
| <I/σ(I)> | 9.81 | 2.1 |
| Completeness [%] | 98.7 | 86.3 |
| Redundancy | 5.3 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% (w/v) PEG 3350; protein was treated with EDTA prior to crystallization, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
