3LWJ

Crystal structure of Putative TetR-family transcriptional regulator (YP_752756.1) from Syntrophomonas wolfei str. Goettingen at 2.07 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-25
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	P 32 2 1
Unit cell lengths	68.184, 68.184, 75.710
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.050 - 2.070
R-factor	0.19
Rwork	0.188
R-free	0.23400
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.434
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.050	59.050	2.140
High resolution limit [Å]	2.070	4.440	2.070
Rmerge	0.078	0.013	0.013
Number of reflections	12714	1400	1150
<I/σ(I)>	17.45	43	2.3
Completeness [%]	99.0	99.6	96.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.3	277	25.0000% Ethylene-Glycol, No Buffer pH 4.3, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K