3LWI
Crystal structure of Cren7-dsDNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-28 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97947 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 77.443, 77.232, 105.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.20489 |
| Rwork | 0.203 |
| R-free | 0.23351 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jtm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.373 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.065 | 0.460 |
| Number of reflections | 15270 | |
| <I/σ(I)> | 39.6 | 4.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.8 | 293 | 30% PEG1500, pH 6.8, VAPOR DIFFUSION, temperature 293.0K |
