3LWG
Crystal structure of HP0420-homologue C46A from helicobacter felis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2009-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.302, 67.129, 70.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.520 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lw3 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.082 | 0.318 |
| Number of reflections | 23847 | |
| <I/σ(I)> | 30.5 | 3.5 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 9.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 287 | 0.01M Cobalt(II)chloride hexahydrate, 0.1M MES pH6.5, 1.8M ammonium sulfate., EVAPORATION, temperature 287K |
