3LW5
Improved model of plant photosystem I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.655, 189.086, 129.388 |
| Unit cell angles | 90.00, 91.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.351 |
| Rwork | 0.349 |
| R-free | 0.38300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wsc |
| RMSD bond length | 0.023 |
| RMSD bond angle | 4.757 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 82447 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 22.5mM MES BIS-TRIS, 10mM SUCCINIC ACID, 6% PEG6000, 0.015% DTM, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
