3LW1
Binary complex of 14-3-3 sigma and p53 pT387-peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.85 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.320, 112.320, 62.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.570 - 1.280 |
| R-factor | 0.128 |
| Rwork | 0.126 |
| R-free | 0.15100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ywt |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.029 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.570 | 1.300 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.088 | 0.579 |
| Number of reflections | 74319 | |
| <I/σ(I)> | 20.8 | 5.1 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 10.6 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1M HEPES, 0.2M calcium chloride, 28% PEG 400, 5% glycerol, 2mM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
