3LV4
Crystal structure of the glycoside hydrolase, family 43 YxiA protein from Bacillus licheniformis. Northeast Structural Genomics Consortium Target BiR14.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.585, 60.327, 73.122 |
| Unit cell angles | 90.00, 97.47, 90.00 |
Refinement procedure
| Resolution | 45.244 - 1.695 |
| R-factor | 0.1486 |
| Rwork | 0.146 |
| R-free | 0.19030 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.056 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.760 |
| High resolution limit [Å] | 1.695 | 1.700 |
| Rmerge | 0.092 | 0.528 |
| Number of reflections | 192259 | |
| <I/σ(I)> | 15.2 | 2.2 |
| Completeness [%] | 97.3 | 92.1 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.15 | 291 | 18% PEG 3350, 0.2M Ca acetate, 0.1M MES, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
