3LUQ
The Crystal Structure of a PAS Domain from a Sensory Box Histidine Kinase Regulator from Geobacter sulfurreducens to 2.5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-01-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.545, 71.971, 67.374 |
| Unit cell angles | 90.00, 113.05, 90.00 |
Refinement procedure
| Resolution | 35.990 - 2.490 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.27900 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.381 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.490 | 2.500 |
| Rmerge | 0.088 | 0.680 |
| Number of reflections | 19104 | |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 98.3 | 97.9 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 289 | 30% PEG 3000, 0.1M CHES pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
