3LU2
Structure of lmo2462, a Listeria monocytogenes amidohydrolase family putative dipeptidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.18072 |
| Spacegroup name | P 61 |
| Unit cell lengths | 179.088, 179.088, 46.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.600 - 2.200 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.557 |
| Number of reflections | 43794 | |
| <I/σ(I)> | 14.6 | 2.5 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 5.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 25% PEG 3350, 200mM Na Tartrate, 100mM Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
