3LTM
Structure of a new family of artificial alpha helicoidal repeat proteins (alpha-Rep) based on thermostable HEAT-like repeats
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.91971, 0.9795, 0.93 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.440, 52.450, 83.970 |
| Unit cell angles | 90.00, 91.37, 90.00 |
Refinement procedure
| Resolution | 19.802 - 2.150 |
| R-factor | 0.1779 |
| Rwork | 0.174 |
| R-free | 0.25030 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Number of reflections | 20242 | |
| <I/σ(I)> | 14.4 | 3.2 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 292 | 18.5% PEG 1000, 6.5% glycerol, 100 mM tricine pH8, 230 mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
