3LTI
Crystal structure of the Escherichia coli RNA polymerase beta subunit beta2-betai4 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X3A |
| Synchrotron site | NSLS |
| Beamline | X3A |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2009-07-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9919 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.314, 52.039, 61.826 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 1.600 |
| R-factor | 0.20884 |
| Rwork | 0.208 |
| R-free | 0.22955 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.134 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0091) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 42737 | |
| <I/σ(I)> | 40 | 5.1 |
| Completeness [%] | 98.5 | 94 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2 M potassium-sodium tartrate, 20% PEG 3350, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
