3LT6
A transition from strong right-handed to canonical left-handed supercoiling in a conserved coiled coil segment of trimeric autotransporter adhesins - the Mutant 4 structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.000, 115.760, 48.080 |
| Unit cell angles | 90.00, 89.93, 90.00 |
Refinement procedure
| Resolution | 19.300 - 1.800 |
| R-factor | 0.22935 |
| Rwork | 0.227 |
| R-free | 0.26619 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.003 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.430 |
| Number of reflections | 37715 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 98.4 | |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | VAPOR DIFFUSION, temperature 293K |
