3LSZ
Crystal structure of glutathione s-transferase from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 167.920, 50.398, 133.228 |
| Unit cell angles | 90.00, 125.51, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| Rwork | 0.206 |
| R-free | 0.22610 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.080 | 0.364 |
| Number of reflections | 95616 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 95.4 | 67.9 |
| Redundancy | 6.7 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 25% PEG 3350; 0.2M Ammonium Acetate; 0.1M Bis-Tris pH 6.5 and glutathione, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
