3LSV
Structure of the A237F mutant of the pentameric ligand gated ion channel from Gloeobacter Violaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.730, 133.008, 159.898 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 20.970 - 3.150 |
| R-factor | 0.2153 |
| Rwork | 0.215 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.222 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER-TNT (2.5.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.150 |
| High resolution limit [Å] | 3.150 | 3.140 |
| Rmerge | 0.153 | 0.600 |
| Number of reflections | 64368 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | PEG 4000 ~25%, NaSCN ~400 mM, NaAc ph 4 100 mM, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
