3LSJ
Crystal structure of DesT in complex with palmitoyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.677, 100.466, 60.838 |
| Unit cell angles | 90.00, 105.15, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.300 |
| R-factor | 0.22551 |
| Rwork | 0.223 |
| R-free | 0.27580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.053 |
| Number of reflections | 21188 |
| <I/σ(I)> | 2.8 |
| Completeness [%] | 98.1 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | ML- 0.1M HEPES, pH 7.5, 0.1 M magnesium acetate, 15% PEG 4000. Drop- 20 mg/ml protein, 0.9 mM 16:0-CoA. 2+2 ul drop., VAPOR DIFFUSION, SITTING DROP, temperature 291K |
