3LSG
The crystal structure of the C-terminal domain of the two-component response regulator yesN from Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.224, 66.224, 245.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.290 - 2.049 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.24040 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.944 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.106 | 0.763 |
| Number of reflections | 39683 | |
| <I/σ(I)> | 26.2 | 2.6 |
| Completeness [%] | 98.0 | 95.3 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 0.2M di-sodium hydrogen phosphate, 20% PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
