3LS1
Crystal Structure of Cyanobacterial PsbQ from Synechocystis sp. PCC 6803 complexed with Zn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-29 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54180 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 92.771, 29.030, 99.503 |
| Unit cell angles | 90.00, 97.77, 90.00 |
Refinement procedure
| Resolution | 21.760 - 1.850 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ls0 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.010 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.760 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.060 | 0.264 |
| Number of reflections | 22914 | |
| <I/σ(I)> | 20.4 | 4.5 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 8.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 25% PEG 1450, 0.1M MES-NaOH, pH 6.5, 0.05M ZINC CHLORIDE, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
