3LRU
hPRP8 Non-Native Subdomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-27 |
| Wavelength(s) | 1.11586 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.732, 42.176, 98.381 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 1.850 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.443 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.10) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 3.990 | 1.850 |
| Rmerge | 0.063 | 0.034 | 0.529 |
| Number of reflections | 30833 | ||
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 99.9 | 99.3 | 99.8 |
| Redundancy | 7.3 | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | vapour diffusion | 6 | 298 | 12% PEG4000, Tris Ph8, 1,3 diamino-propane, pH 6, vapour diffusion, temperature 298K |
