3LRP
Crystal Structure of Plasmodium falciparum ADP-Ribosylation Factor 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9860 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 45.732, 147.280, 64.229 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.120 - 2.500 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hur |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.120 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.092 | 0.017 |
| Number of reflections | 7290 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 92.7 | 84.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 1.5 M ammonium sulfate, 5 mM magnesium chloride, Tris-HCl buffer, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
