3LRE

Crystal Structure Analysis of Human Kinesin-8 Motor Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2004-04-12
Detector	BRUKER AXS PROTEUM/R6000
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	69.420, 79.744, 140.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.710 - 2.200
R-factor	0.223
Rwork	0.221
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t5c
RMSD bond length	0.020
RMSD bond angle	1.699
Data reduction software	PROTEUM PLUS
Data scaling software	SAINT
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	70.710
High resolution limit [Å]	2.200
Number of reflections	39803
<I/σ(I)>	6.9
Completeness [%]	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.8	275	10-13% PEG 20000, 0.1M HEPES pH 7.8, 2% dioxane, vapor diffusion, temperature 275K