3LRD
Self-assembly of spider silk proteins is controlled by a pH-sensitive relay
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.383, 68.383, 97.781 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.200 - 2.150 |
| R-factor | 0.17299 |
| Rwork | 0.170 |
| R-free | 0.22841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NT wild-type |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.423 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.230 | 2.206 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.087 | 0.421 |
| Number of reflections | 14086 | |
| <I/σ(I)> | 7.2 | 4 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | Ammonium sulphate, PEG 400, VAPOR DIFFUSION |
