3LRA

Structural Basis for Assembling a Human Tripartite Complex Dlg1-MPP7-Mals3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-12-30
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 62 2 2
Unit cell lengths	153.190, 153.190, 58.903
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.300 - 2.950
R-factor	0.255
Rwork	0.255
R-free	0.28400
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	3.060
High resolution limit [Å]	2.950	2.950
Rmerge	0.092	0.435
Number of reflections	8045
<I/σ(I)>	25.7	2.1
Completeness [%]	95.0	77.7
Redundancy	14.8	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	10% PEG 8000, 0.2M MgCl2, 0.1M Guanidine HCl, 0.1M Tris-HCl, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	7	293	10% PEG 8000, 0.2M MgCl2, 0.1M Guanidine HCl, 0.1M Tris-HCl, pH7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K