3LR2

Self-assembly of spider silk proteins is controlled by a pH-sensitive relay

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 32 2 1
Unit cell lengths	68.379, 68.379, 97.857
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.200 - 1.700
R-factor	0.163
Rwork	0.160
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.366
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.230	1.744
High resolution limit [Å]	1.700	1.700
Rmerge		0.060
Number of reflections	27832
<I/σ(I)>		8.4
Redundancy		5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	ammonium sulphate, PEG 400, VAPOR DIFFUSION, temperature 293K
1	VAPOR DIFFUSION		293	ammonium sulphate, PEG 400, VAPOR DIFFUSION, temperature 293K