3LR0

Periplasmic domain of the risS sensor protein from Burkholderia pseudomallei, iodide phased at low pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-10
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	58.360, 58.360, 75.690
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.100 - 1.900
R-factor	0.213
Rwork	0.211
R-free	0.25400
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.388
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.100	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.337
Number of reflections	12191	924
<I/σ(I)>	25.45	4.1
Completeness [%]	99.8	99.9
Redundancy	11.2	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	289	Crystal originally grown in JCSG+ condition a6, 0.1M phosphate-citrate pH 4.2, 0.2 M lithium sulfate, 25% PEG 1000 then soaked and cryo-protected into 0.1 M Na citrate pH 4.5, 1.0 M KI, 30% PEG 1000 for 1 hour prior to vitrification; crystal tracking IDs 203087a6 for growth and 206239a1 for soak; affinity tag not removed prior to crystallization, VAPOR DIFFUSION, SITTING DROP, temperature 289K