3LQR
Structure of CED-4:CED-3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 181.330, 181.330, 202.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.776 - 3.896 |
| R-factor | 0.2569 |
| Rwork | 0.255 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a5y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.581 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.776 | 4.040 |
| High resolution limit [Å] | 3.896 | 3.896 |
| Rmerge | 0.077 | 0.883 |
| Number of reflections | 15624 | |
| <I/σ(I)> | 18 | 1.75 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M Tris-HCl, pH 8.5, 0.6-0.8M potassium sodium tartrate tetrahydrate, 0.1M guanidine-HCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
