3LQN
Crystal Structure of CBS Domain-containing Protein of Unknown Function from Bacillus anthracis str. Ames Ancestor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-21 |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 27.585, 120.770, 80.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.400 - 1.800 |
| R-factor | 0.1864 |
| Rwork | 0.184 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2emq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.636 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 12662 | |
| <I/σ(I)> | 23.9 | 5.5 |
| Completeness [%] | 98.2 | 81.9 |
| Redundancy | 8.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | 277 | 0.2M litium sulphate, 0.1 M CAPS pH 10.5, 2.0 M ammonium sulphate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
