3LQE
X-Ray Structure of the Murine Norovirus (MNV)-1 Capsid Protein Protruding (P) Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-07-31 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97856 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 86.262, 105.219, 84.053 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.840 - 2.000 |
R-factor | 0.2175 |
Rwork | 0.215 |
R-free | 0.25620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | crystal structure (in house) |
RMSD bond length | 0.026 |
RMSD bond angle | 2.431 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.071 | |
Number of reflections | 24822 | |
<I/σ(I)> | 10 | 5 |
Completeness [%] | 86.0 | 72 |
Redundancy | 4.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30% PEG 4000, 100 mM Tris, 20 mM sodium chloride, 200 mM lithium sulfate, , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |