3LQD
Crystal structure determination of Lepus europaeus 2.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-11-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.457, 54.954, 91.380 |
| Unit cell angles | 90.00, 97.70, 90.00 |
Refinement procedure
| Resolution | 29.350 - 2.800 |
| R-factor | 0.22541 |
| Rwork | 0.219 |
| R-free | 0.27905 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rao |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.575 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.141 | 0.318 |
| Number of reflections | 13388 | |
| <I/σ(I)> | 0.9 | |
| Completeness [%] | 90.1 | 92.8 |
| Redundancy | 2.73 | 2.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 293 | 45 % PEG 3350, 50mM Tris-Hcl buffer, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
