3LQB

Crystal structure of the hatching enzyme ZHE1 from the zebrafish Danio rerio

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-02
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	32.970, 62.680, 87.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.980 - 1.100
R-factor	0.16946
Rwork	0.169
R-free	0.18722
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ast
RMSD bond length	0.011
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.100
Rmerge	0.037
Number of reflections	72995
<I/σ(I)>	26.1
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1M MES-NaOH buffer, 20%(w/v) PEG 3350, 0.2M potassium sulphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6	277	0.1M MES-NaOH buffer, 20%(w/v) PEG 3350, 0.2M potassium sulphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K