3LQ6
Crystal Structure of Murine Norovirus Protruding (P) Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.800, 86.326, 83.543 |
| Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
| Resolution | 35.460 - 2.000 |
| R-factor | 0.191 |
| Rwork | 0.189 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2obr |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.367 | |
| Number of reflections | 49163 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 90.4 | 71 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 20% PEG 3350, 0.2 M Sodium Acetate, 90 mM glycine, 10 mM sodium chloride, 10 mM Tris, 0.5 mM dithiothreitol, , pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
