3LPZ
Crystal structure of C. therm. Get4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97550 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.920, 79.680, 94.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.510 - 1.980 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.22200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.282 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.920 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.098 | 0.474 |
| Number of reflections | 29415 | |
| <I/σ(I)> | 5.7 | 2.7 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 100 mM MES (pH 6.0), 1 M Lithium chloride, and 10% (w/v) PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
