3LPW
Crystal structure of the FnIII-tandem A77-A78 from the A-band of titin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-20 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.900, 163.200, 65.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.805 - 1.650 |
| R-factor | 0.1793 |
| Rwork | 0.179 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | A170 extracted from PDB entry 2NZI |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.019 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.805 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 74213 | |
| <I/σ(I)> | 11.9 | 3.6 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | VAPOR DIFFUSION, HANGING DROP, temperature 277K |
