3LPE

Crystal structure of Spt4/5NGN heterodimer complex from Methanococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	88
Detector technology	PIXEL
Collection date	2009-06-21
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	64.360, 63.970, 83.040
Unit cell angles	90.00, 106.98, 90.00

Refinement procedure

Resolution	44.355 - 1.900
R-factor	0.21
Rwork	0.208
R-free	0.25490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ryq 3ewg
RMSD bond length	0.008
RMSD bond angle	1.039
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.400	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.046	0.600
Number of reflections	47050
<I/σ(I)>		1.4
Completeness [%]	92.1	86.4
Redundancy	1.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	20-30% PEG 400, 50mM MES pH 5.5-7.0, 30mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 295K