3LPD
Crystal structure of a subtilisin-like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.936, 45.655, 47.248 |
| Unit cell angles | 98.00, 115.13, 113.41 |
Refinement procedure
| Resolution | 24.108 - 2.100 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lpc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.229 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.108 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.560 | |
| Number of reflections | 15131 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 93.6 | |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Sodium Acetate, Sodium Cacodylate, PEG 8000, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
