3LPC
Crystal structure of a subtilisin-like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.675, 45.821, 45.746 |
| Unit cell angles | 98.40, 113.98, 114.61 |
Refinement procedure
| Resolution | 20.380 - 1.700 |
| R-factor | 0.137 |
| Rwork | 0.135 |
| R-free | 0.17600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dbi |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.255 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.669 | 20.380 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.048 | 0.030 | 0.223 |
| Total number of observations | 3766 | 15918 | |
| Number of reflections | 26960 | ||
| <I/σ(I)> | 22.6 | 21 | 3.4 |
| Completeness [%] | 92.0 | 96.2 | 88 |
| Redundancy | 4.2 | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Sodium Acetate, Sodium Cacodylate, PEG 8000, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
