3LPA
Crystal structure of a subtilisin-like protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.059, 45.980, 47.243 |
| Unit cell angles | 97.79, 115.24, 113.86 |
Refinement procedure
| Resolution | 24.260 - 2.000 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lpc |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.343 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.21) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.659 | 30.470 | 2.110 |
| High resolution limit [Å] | 2.000 | 6.320 | 2.000 |
| Rmerge | 0.060 | 0.033 | 0.265 |
| Total number of observations | 1339 | 5642 | |
| Number of reflections | 17666 | ||
| <I/σ(I)> | 13.5 | 16.6 | 2.7 |
| Completeness [%] | 93.9 | 97.1 | 89.3 |
| Redundancy | 2.3 | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Sodium Acetate, Sodium Cacodylate, PEG 8000, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
