3LP9
Crystal structure of LS24, A Seed Albumin from Lathyrus sativus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.170, 88.140, 154.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.630 - 2.200 |
| R-factor | 0.24662 |
| Rwork | 0.244 |
| R-free | 0.26600 |
| Structure solution method | MIR |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.963 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.129 | 0.491 |
| Total number of observations | 185883 | |
| Number of reflections | 54633 | |
| <I/σ(I)> | 5.2 | 1.9 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 10% (w/v) PEG 200000, 0.1M MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
