3LP5
The crystal structure of the putative cell surface hydrolase from Lactobacillus plantarum WCFS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.680, 122.154, 38.659 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.080 - 2.000 |
| R-factor | 0.17768 |
| Rwork | 0.175 |
| R-free | 0.23647 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.728 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.080 | 2.052 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.187 | 0.665 |
| Number of reflections | 16267 | |
| <I/σ(I)> | 15.36 | 1.3 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 8.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 1.6M tri-sodium citrate dehydrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
