3LP1
HIV-1 reverse transcriptase with inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC/MAR |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 118.784, 155.264, 155.244 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.230 |
| R-factor | 0.26906 |
| Rwork | 0.268 |
| R-free | 0.29217 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.962 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.310 |
| High resolution limit [Å] | 2.230 | 4.800 | 2.230 |
| Rmerge | 0.049 | 0.038 | 0.251 |
| Number of reflections | 67667 | ||
| <I/σ(I)> | 15.2 | ||
| Completeness [%] | 96.3 | 86.4 | 97.2 |
| Redundancy | 5.1 | 5.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 6.8 | 298 | 0.1 M Sodium cacodylate pH 6.8, 0.8 M Sodium Citrate, sitting drop, temperature 298K |
