3LOW

Crystal structure of Beta 2 Microglobulin domain-swapped dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Wavelength(s)	0.97166
Spacegroup name	P 1 21 1
Unit cell lengths	59.721, 29.162, 67.715
Unit cell angles	90.00, 97.49, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.222
Rwork	0.219
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.595
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.140	50.000	2.230
High resolution limit [Å]	2.190	5.940	2.190
Rmerge	0.057	0.038	0.205
Number of reflections	12176
<I/σ(I)>	18.3
Completeness [%]	99.3	97.4	99.1
Redundancy	3.6	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1